Iris Bellinghausen, PhD,a,b* Benno Weigmann, PhD,c* Victor Zevallos, PhD,b,d Joachim Maxeiner, MSc,e
Sonja Reißig, PhD,b,f Ari Waisman, PhD,b,f Detlef Schuppan, MD, PhD,b,d* and Joachim Saloga, MDa,b*
Background: Amylase-trypsin inhibitors (ATIs) in wheat and related cereals are potent activators of myeloid innate immune cells via engagement of TLR4. Furthermore, ATIs have been shown to serve as adjuvants in experimental intestinal inflammatory diseases .
Objective: The aim of this study was to analyze whether ATIs are also modifiers of allergic inflammation.
Methods: Therefore, CD4+ T cells from donors sensitized to grass or birch pollen were stimulated with autologous allergen-pulsed dendritic cells in the presence or absence of ATIs or the control storage protein zein from corn. To analyze allergen-induced gut and lung inflammation, immune deficient mice were engrafted with PBMCs from these allergic donors plus the respective allergen, and fed with selected diets. Three weeks later, inflammation was induced by rectal or intranasal allergen challenge and monitored by mini endoscopy or airway hyperreactivity, respectively.
Results: Allergen-specific T-cell proliferation and cytokine production was significantly exacerbated by ATIs and not by zein. In vivo, allergen-specific human IgE level was strongly elevated in sera of mice receiving an ATI-containing diet compared with mice that were fed gluten-free and thus ATI-free diet. Importantly, allergen-induced IgE-dependent colitis and airway hyperreactivity were also enhanced in ATI-fed mice. Gut inflammation was further increased in mice receiving an additional ATI injection and even detectable in the absence of the aeroallergen, whereas zein had no such effect. Injection of anti-human TLR4 mAbs or the anti-human IgE mAb omalizumab completely abolished ATI-induced allergic inflammation.
Conclusions: These results underline that wheat ATIs are important nutritional activators and adjuvants of allergy, which might be exploited for nutritional therapeutic strategies.
報告順序：20191206-1
組員：生技碩二 孫筱瑋 7107041044
生技碩一 林宜臻 7108041031
指導教授：徐堯煇、王敏盈 教授
